Authors: Laura Clarke, Xiangqun Zheng-Bradley, Richard Smith, Eugene Kulesha, Chunlin Xiao, Iliana Toneva, Brendan Vaughan, Don Preuss, Rasko Leinonen, Martin Shumway, Stephen Sherry & Paul Flicek (Source: Nature Methods)
Authors: John R Yates, Sung Kyu Robin Park, Claire M Delahunty, Tao Xu, Jeffrey N Savas, Daniel Cociorva & Paulo Costa Carvalho
Informatics has driven mass spectrometry–based protein analysis to create large-scale methods for proteomics. As software algorithms have developed, comparisons between algorithms are inevitable. We outline steps for fair and objective comparisons that will make true innovations apparent. (Source: Nature Methods)
Authors: John S Oliver & Valentin Dimitrov
Using semiconductor processing to construct integrated circuits that reside close to nanopores, researchers demonstrate high-bandwidth, low-noise measurements of DNA translocation through solid-state nanopores. (Source: Nature Methods)
Authors: Gauri Kulkarni & William G Wadsworth
A strategy that uses genetically encoded GFP-tagged antibodies allows in vivo imaging of extracellular non–genetically encoded molecules. (Source: Nature Methods)
Authors: Jiekai Chen & Duanqing Pei
Current practice for the generation and maintenance of induced pluripotent stem cells (iPSCs) involves static culture in dishes. Two groups now report that mouse iPSCs can be generated efficiently in stirred suspension culture. (Source: Nature Methods)
Nature Methods 9, 443 (2012).
doi:10.1038/nmeth.1979
Author: Monya Baker
Light and chemicals offer precise ways to manipulate proteins. (Source: Nature Methods)
Nature Methods 9, 440 (2012).
doi:10.1038/nmeth.2009
Author: Monya Baker
Proteomics analysis of polyclonal antibodies guides monoclonal production. (Source: Nature Methods)
Nature Methods 9, 438 (2012).
doi:10.1038/nmeth.2008
Author: Tal Nawy
A simple microfluidic device allows long-term imaging of single budding yeast cells. (Source: Nature Methods)
Nature Methods 9, 437 (2012).
doi:10.1038/nmeth.2007
Author: Nicole Rusk
Downregulation of natural antisense transcripts enhances expression of their sense counterparts. (Source: Nature Methods)
Nature Methods 9, 436 (2012).
doi:10.1038/nmeth.1998 (Source: Nature Methods)